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Research > Research Cores > Flow Cytometry and Fluorescence Activated Cell Sorting

Home | Introduction to Flow Cytometry | FACS FAQs | High-Speed Cell Sorting | LSR II and Calibur | Fees | Login

The Flow Cytometry Core provides investigators with instrumentation and support for cell sorting as well as acquisition and analysis of flow cytometry data. Services offered:

  • Staff-operated cell sorting

  • Assistance with experimental design

  • Instruction and training on the instruments

  • Consultations on sample preparation and data analysis 

Following instruments are available in the Flow Cytometry Core:

Two BD FACSAriaII cell sorters from BD Biosciences;

All sorters provide high-speed, high purity multi-parameter cell sorting into tubes and are also capable of single cell sorting directly into 96-well plates. BD FACSAriaII-2 cell sorter is available for operation by trained users and BD FACSAriaII-1 is operated by the core staff only. 
 
BD LSR II, BD FACSCanto II and  BD FACSCalibur analyzers;

These instruments allow analysis of up to 15 fluorescent parameters. New users are provided with training before being able to operate the instruments.
Use the link at the bottom of this page to access the instrument schedule and make appointments.

Every Flow Cytometry Center user needs to have an individual account.
Pathology and Immunology Department members should login with their Network username and password.
First time users not from the Department of Pathology and Immunology please follow this link to register.
When you log in first time fill in all required information and request access to instruments.
Please allow one business day for the activation of your account.
Contact the Facs Staff if you have any questions or problems with the new account.


Two analysis workstations equipped with FlowJo, BD FACS Diva and FCAP Array analysis software are available at no charge.

Please use only polystyrene tubes on all analyzers including LSRII
(BD Falcon tubes cat# 352008)

FlowJo site license

 FACS core has acquired a site license for FlowJo. Any lab can get a Serial Number for one computer for $100 a year. The cost for each additional computer is $250. This results in the significant discount compared to individual purchase.     
 
Contact
               Director: Marina Cella, MD
               Lab Manager/Cell Sorting Operator: Erica Lantelme, PhD
               Cell Sorting Operator: Dorjan Brinja
Phone: (314) 362-3562
Location: 8513 BJCIH Building
Email: facs@pathology.wustl.edu