Research > Research Cores > Flow Cytometry and Fluorescence Activated Cell SortingHome | Introduction to Flow Cytometry | FACS FAQs | High-Speed Cell Sorting | LSR II and Calibur | Fees | Login
The Flow Cytometry Core provides investigators with instrumentation and support for cell sorting as well as acquisition and analysis of flow cytometry data. Services offered:
Staff-operated cell sorting
Assistance with experimental design
Instruction and training on the instruments
Consultations on sample preparation and data analysis
Every Flow Cytometry User needs to have an individual account. First time users please follow this link to register. When you log in first time fill in all required information and request access to instruments. Please allow one business day for the activation of your account. Contact the Facs Staff if you have any questions or problems with the new account (email@example.com).
INSTRUMENTS available in the Flow Cytometry Core:
BD FACSAriaII CELL SORTERS. All sorters provide high-speed, high purity multi-parameter cell sorting into tubes (15ml, 5ml or 1.5ml) or sorting of single or multiple cells directly into 96-well plates.
BD FACSAriaII-1: operated by the core Staff only. Lasers: 355nm, 405nm, 488nm, 561nm, 633nm. Up to 14 colors detected
BD FACSAriaII-2: available for operation by trained users. Lasers: 405nm, 488nm, 633nm. Up to 10 colors detected
BD FACSAriaII-3: available in BSL2+. Lasers: 405nm, 488nm, 561nm, 633nm. Up to 11 colors detected. Recommended for sorting of infected animal cells, clinical samples when testing uncertain, yeast and bacteria. Long cleaning is required in specific circumstances.
BD FACS ANALYZERS: New users are provided with training before being able to operate the instruments. Please use only polystyrene tubes on all analyzers including LSRII (BD Falcon tubes cat# 352008). CSTs are performed every week on all the instruments to grant optimal performance.
LSRII: Lasers: 355nm, 405nm, 488nm, 561nm, 633nm. Up to 15 colors detected. BD High Throughput Sampler (HTS) available for 96 well plates.
LSR FORTESSA: Lasers: 405nm, 488nm, 561nm, 633nm. Up to 13 colors detected.
CantoII: Lasers: 405nm, 488nm, 633nm. Up to 8 colors detected.
FACSCaliburs-1and Calibur-3: Lasers: 488nm, 633nm. Up to 4 colors detected.
SEAHORSE BIOFLUX ANALYZERS XF96 and XF24. Available for operation by trained users.
Two analysis workstations equipped with FlowJo, BD FACS Diva and FCAP Array analysis software are available at no charge.
FlowJo site license. FACS core has acquired a site license for FlowJo.
The cost for the license is $250 per billing year (Oct 1st- Sept 30th). The first licence is offered at the discounted price of $100. This results in the significant discount compared to individual purchase. If you have any questions about FlowJo please contact Erica Lantelme (firstname.lastname@example.org)
Contact : Director: Marina Cella, MD (email@example.com)
Lab Manager/Cell Sorting Operator: Erica Lantelme, PhD (firstname.lastname@example.org)
Cell Sorting Operator: Dorjan Brinja (email@example.com)
Location: BJC-IH Building, 8th floor, Room 8513-8518
Phone: (314) 362-3562