Research > Research Cores > Hybridoma Center
Monoclonal Antibody Development Core

The Washington University School of Medicine Hybridoma Center (WUHC) was established in 1979 to provide monoclonal antibody technology to the University research community. The Hybridoma Center assists investigators in the generation of monoclonal antibody-producing B cell hybridomas. Initially, guidance is provided by the Director in the custom selection of appropriate species to be used, development of immunization schedules, screening protocols, and methods for antibody production. Immunized spleen cells derived from either mice or Armenian hamsters are fused and maintained at the Hybridoma Center. Once sufficient growth has occurred, culture supernatants are screened by ELISA and/or provided to the investigator for screening. Hybridomas secreting antigen-specific antibodies are cloned to establish a stable line secreting a unique antibody with defined specificity and isotype. After a second round of cloning, the antibody producing cultures are expanded and returned to the investigator for further processing. Frozen vials are maintained in liquid nitrogen by the Hybridoma Center.

The WUHC also provides custom monoclonal antibody development for parties outside of the university. Contact the director for further details.

Hybridoma Center Links

Hybridoma Center Guidelines
Directions to the Hybridoma Center
Immunization Protocol
General Fusion Protocol
Hybridoma Center Publications
Hybridoma Production Flow Chart
Multi-Tiered Screening Protocol
Applications of Monoclonal Antibodies
Advantages and Disadvantages of Monoclonal Antibodies
Advantages of Armenian hamster Monoclonal Antibodies

Antibody Resource Links

Linscott's Directory
Antibody Resource Page
Developmental Studies Hybridoma Bank: University of Iowa
Conjugation of Monoclonal Antibodies

For more information:

Director: Kathleen Sheehan, PhD
Staff: Misha Hart & Paul Schjetnan
Location: 4724 West Building, Campus Box 8118
Consult & Pricing: (314) 362-8744
Scheduling & Data Analysis: (314) 362-7448
Fax: (314) 747-1227