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         | Research > Research Cores > Hybridoma Center Monoclonal Antibody Development Core The Washington University School of Medicine Hybridoma Center (WUHC) was established in 1979 to provide monoclonal antibody technology to the University research community. The Hybridoma Center assists investigators in the generation of monoclonal antibody-producing B cell hybridomas. Initially, guidance is provided by the Director in the custom selection of appropriate species to be used, development of immunization schedules, screening protocols, and methods for antibody production. Immunized spleen cells derived from either mice or Armenian hamsters are fused and maintained at the Hybridoma Center. Once sufficient growth has occurred, culture supernatants are screened by ELISA and/or provided to the investigator for screening. Hybridomas secreting antigen-specific antibodies are cloned to establish a stable line secreting a unique antibody with defined specificity and isotype. After a second round of cloning, the antibody producing cultures are expanded and returned to the investigator for further processing. Frozen vials are maintained in liquid nitrogen by the Hybridoma Center. Hybridoma Center Guidelines Antibody Resource Links Linscott's Directory For more information: Director: Kathleen Sheehan, PhD | |
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